Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF.

نویسندگان

  • E K Sullivan
  • C S Weirich
  • J R Guyon
  • S Sif
  • R E Kingston
چکیده

Chromatin remodeling complexes such as SWI/SNF use the energy of ATP hydrolysis to remodel nucleosomal DNA and increase transcription of nucleosomal templates. Human heat shock factor one (hHSF1) is a tightly regulated activator that stimulates transcriptional initiation and elongation using different portions of its activation domains. Here we demonstrate that hHSF1 associates with BRG1, the ATPase subunit of human SWI/SNF (hSWI/SNF) at endogenous protein concentrations. We also show that hHSF1 activation domains recruit hSWI/SNF to a chromatin template in a purified system. Mutation of hHSF1 residues responsible for activation of transcriptional elongation has the most severe effect on recruitment of SWI/SNF and association of hHSF1 with BRG1, suggesting that recruitment of chromatin remodeling activity might play a role in stimulation of elongation.

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عنوان ژورنال:
  • Molecular and cellular biology

دوره 21 17  شماره 

صفحات  -

تاریخ انتشار 2001